elastase from human leukocytes
elastase from human leukocytes
CAS: 9004-06-2
Molecular Formula:
elastase from human leukocytes - Names and Identifiers
elastase from human leukocytes - Physico-chemical Properties
| Appearance | lyophilized powder |
| Storage Condition | -20°C |
| Physical and Chemical Properties | Elastase is white or light gray crystalline powder. It is soluble in water, stable at ph4 ~ 10.5 and 20C, and can be stored for 3~12 months at 60C. Compound elastic ointment enzyme is a white ointment containing elastase and new mildew sulfate. |
| Use | For the regulation of blood lipids |
elastase from human leukocytes - Risk and Safety
| WGK Germany | 3 |
| FLUKA BRAND F CODES | 3-10 |
elastase from human leukocytes - Nature
Open Data Verified Data
- This product is white or light gray crystalline powder. Hygroscopic, soluble in water. Stable at pH 4~10.5 and 2 ℃, pH>6 can prolong the stability time. Frozen Dry powder can be stored at 5 degrees C for 3 to 12 months. The molecular weight was 25900 and the isoelectric point was pl = 9.5.
- pure pancreatic elastase consists of a single peptide chain of 240 amino acid residues with 4 pairs of disulfide bonds in the molecule. The peptide chain and the spatial configuration are very similar to the chymotrypsin, and the molecule is spherical at pH 5, and there are two a helical regions in the molecule. It is a pure protease, no prosthetic group and metal ions, no allosteric center, its activity depends on the specific three-dimensional structure. The active center amino acid residues are: group 45. Day 93. Silk 88, the amino acid residues in the vicinity of the reactive serine are arranged in the order: one gan · day · silk · gan 1.
- elastase can hydrolyze hemoglobin and fibrin in addition to elastin, and has no effect on hair protein. Many inhibitors can reduce or eliminate the activity of elastase, such as 10-5 mol.L-1 copper sulfate, 7 × 10-2 mo I .L-1 sodium chloride can inhibit 50% enzyme activity, sodium cyanide, ammonium sulfate, potassium chloride, phosphorus trichloride also has a similar effect, generally reversible, soybean trypsin inhibitor, serum or intestinal non-dialysate, etc. also have an inhibitory effect. Others, such as thiomalic acid, mercaptosuccinic acid, diisopropyl fluorophosphoric acid, etc., can strongly inhibit the enzyme activity.
Last Update：2024-01-02 23:10:35
elastase from human leukocytes - Preparation Method
Open Data Verified Data
fresh or frozen porcine pancreas was minced, 3 times the amount of acetone below 0 ° C. Was added, and th was stirred, centrifuged, and the precipitate was separated. The wet cake was further added with 3 times the amount of cold acetone, and the above operation was repeated. Then, the wet cake was dried in a vacuum drying oven and pulverized to obtain a dry Acetone Powder. Add 20 times of water to the acetone dry powder, stir and extract at room temperature for 2H, add a small amount of talc to filter the extract when filtering, and obtain a clear extract after filtering. The extract was diluted to 2-3 times with water and added with 0. Imol/L, pH6.4 phosphate buffer solution equilibrium Amberlite CG-50 resin (raw material: resin 1:2), stirring adsorption 2H, or 445 macroporous resin (extract solution: pH 4.5 acetate buffer solution): resin -30:1), stirred to adsorb th. The resin was rinsed again with distilled water. Then, 1.5 mol/L, ph 9.3 ammonium chloride buffer (resin: Buffer -1:) was added to the resin, and th was eluted by stirring, and the resin was separated. The eluent was adjusted to pH neutral with 2mol/L acetic acid, and filtered to obtain a clear eluent. The enzyme on the 445 macroporous resin can be eluted by ammonium chloride solution at pH CG-50, and the elution conditions can also be similar to those of Amberlite resin. The eluent was added with 3 times the amount of acetone at -5 °c under stirring at 5 °c; Stirring was continued for 10 min, and the precipitate was allowed to stand at -5 °c for several hours. The precipitate was collected by filtration, washed with acetone and diethyl ether for 2-3 times, and dried under vacuum to obtain elastase.
Last Update：2022-01-01 11:10:54
elastase from human leukocytes - Use
Open Data Verified Data
- enzyme preparation can hydrolyze the N-terminal peptide bonds of aliphatic amino acids on protein molecules, and the proteins that can be hydrolyzed include special elastin and cellulose, hemoglobin, albumin and casein on elastic fibers. It can improve the abnormal lipid metabolism in serum and tissues, promote the dissimilation and excretion of cholesterol in liver, increase the activity of lipoprotein, and promote the metabolism of neutral fat and lipoprotein, especially very low density lipoprotein and low density lipoprotein; can inhibit the occurrence of atherosclerosis and inhibit the formation of experimental liver fibrosis and fatty liver.
- preparation elastase lyophilized injection: 15mg/vial; Enteric-coated tablets: 10 mg (200u)/tablet; Enteric-coated capsules: 6mg/pill; Enteric-coated pills: 10 mg/pill.
- usage and dosage for clinical use (1) hyperlipidemia: enteric-coated capsules, 6mg each time. Enteric-coated tablets, 10~20mg each time, 3 times a day. Or powder injection, intramuscular injection, Daily 15mg,3 months for a course of treatment;(2) Chronic bronchitis: Enteric coated capsules, 12mg each time, 3 times a day, a month for a course of treatment. (3) atherosclerosis, hypertension, fatty liver: dose with hyperlipidemia.
Last Update：2022-01-01 11:10:55
elastase from human leukocytes - Safety
Open Data Verified Data
- he has occasional allergic and mild gastrointestinal symptoms, with abdominal distension, loss of appetite, liver pain, dry mouth and numbness of lips. No need for treatment, can be self-healing.
- sealed with shading and stored at 2 ℃ or less.
Last Update：2022-01-01 11:10:55
elastase from human leukocytes - Reference Information
| EPA chemical substance information | information provided by: ofmpeb.epa.gov (external link) |
| Overview | More than 100 years ago it was known that there are elastic enzyme activity in the arteries and pancreas, but for a long time, it is considered to be trypsin, and pancreatic elastase was isolated from Balo and Banga until 1949. since then, it has been studied for its physiological action and pharmacological effect, and is clinically used for the treatment of hyperlipidemia, atherosclerosis and fatty liver. Japan was formally identified as a drug supply market in 1981. Elastase is the most striking feature is the dissolution of elastin, at the beginning people think that elastase is not a single enzyme, it contains elastic hydrolysis (elastic hydrolysis), viscous (hydrolysis) and lipid (hydrolysis) the role of a mixture of three enzymes, respectively, called elastase, elastic mucopolysaccharide enzyme and elastase. More recently, elastase is considered to contain two enzymes: elastase I, panereopolypeptide E (Ec. 3.4.4.7), commonly known as elastase, and elastase II, a newly discovered protease. Elastase is a serine-containing protease, which is composed of 240 amino acids and has a molecular weight of 25900. Its molecular peptide chain orientation and spatial configuration are similar to those of chymotrypsin, it does not contain a prosthetic group and metal ions, its active center depends on the specific three-dimensional structure, when its denaturation, configuration change, it causes inactivation. It is present in the pancreas of all animals as an inactive precursor (elastase). In the human aorta, skin, platelets and white blood cells also exist in the elastic enzyme activity of the material, in addition, some microorganisms and plants also have elastic enzyme activity of material. The elastase substrate has a wide specificity. It can dissolve most proteins, such as blood protein, albumin, casein, fibrin, soybean globulin, etc., in addition to dissolving elastin, but has no effect on hair keratin. In addition, it is also capable of decomposing many substrates for polypeptide synthesis. Elastase is involved in lipid metabolism in blood and arterial vessels in the human body. Animal experiments show that elastase has anti-arteriosclerosis effect and selective effect on denatured elastin, which can prevent the aorta, the formation of atherosclerotic plaque in coronary artery, enhance myocardial anti-hypoxia ability, protect ischemic myocardium, increase coronary flow, promote liver glycogen accumulation, prevent the formation of fatty liver, can prevent cholesterol synthesis and make it into bile acid and excretion, and significant decomposition of chylomicrons, promote lipid clearance from the blood without deposition in the arterial wall; can also reduce the urine beta 2-small globulin, inhibit the renal sphere basement membrane hypertrophy, prevent diabetic kidney disease; Can also reduce the total fat of fatty liver, inhibit the development of fatty liver; Can dissolve collagen, inhibition of lung tissue collagen increase, inhibition of bleomycin treatment side effects-pulmonary fibrosis; Can indirectly affect the activity of plasmin vasopressin; Can also be used for deformation of joint inflammation. In the clinical treatment of hyperlipidemia, diabetes and liver, lung disease. |
| pharmacological effects | an enzyme in this strain that dissolves elastin, pancreatic elastase is a polypeptide consisting of 240 amino acids, extracted from the pancreas of animals or produced by fermentation of microorganisms. This enzyme can prevent cholesterol synthesis in the body, and promote its conversion into bile acid, lower blood cholesterol, triglyceride, low density lipoprotein and very low density lipoprotein, increase high density lipoprotein, in addition, this product also promotes blood coagulation, strengthens the effect of uterine contractions. |
| uses | elastase preparations are mainly used to improve abnormal lipid metabolism in serum and tissues, promotes the dissimilation and excretion of cholesterol in the liver; Increases the activity of lipoproteinases, promotes the metabolism of neutral lipids and lipoproteins, especially very low density lipoproteins and low density lipoproteins. Can inhibit the occurrence of atherosclerosis and inhibit the formation of experimental liver fibrosis and fat. But occasional allergies and mild gastrointestinal symptoms used to regulate blood lipids |
| production method | remove fire, wash fat, dry fresh or frozen pig pancreas, put it into a meat grinder, add 3 times the amount of acetone cooled to below 00C, under constant stirring at about 00C dehydration for 1H, centrifugation (waste acetone recovery treatment), separation of precipitation, 3 times the amount of cold acetone is added to the wet cake, and the same operation as above is carried out. The wet cake is dried in a vacuum drying oven and pulverized to obtain acetone dry powder. Cold sealed storage, standby. Crushed pig pancreas acetone (cold) → dehydration; Degreasing; Dry Acetone Powder extraction acetone dry powder add a certain amount of water according to the weight of Acetone Powder, make the weight volume ratio of 5%, under constant stirring, the extract is extracted at 20-250C for 2H, a small amount of talc is added during the filtration of the extract to help the filtration below 200C, and the cotton cloth is filtered naturally or by plate and frame pressure filtration to obtain a clear extract. Acetone Powder water: 200C → extract adsorption extract added a certain amount of distilled water diluted to 2-3 times, added to the pH 6.4 phosphate buffer (0.1mol/L) amberlite CG-50 resin m (raw material):m (resin) = 1: 2, under constant stirring, 20 ~ 25C adsorption 2H, the resin was rinsed with distilled water until the eluent was colorless to give an adsorbate. Extract solution Amberliet CG-50 resin → adsorbate elution 1 part of adsorption resin added 1.5 times ph9.3 ammonium chloride buffer (0.5mol/L), under constant stirring, elution 1H, separation resin, the eluent was adjusted to ph neutral with acetic acid (2mol/L) and filtered to obtain a clear eluent. Adsorbate Ph 9.3 buffer → elution eluent precipitate under-50C, add 3 times of acetone cooled to-50C to the eluent while stirring, continue stirring for 10min, at-50C, the patient was allowed to settle for several hours. Eluent acetone;-50C → precipitation precipitation dehydration, drying the precipitate was collected, washed 3 times with acetone, then washed 3 times with ether, and dried under vacuum to obtain elastase original powder. Acetone precipitation; Diethyl ether → dehydration; Dried elastase raw powder |
Last Update：2024-04-10 22:41:03
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Product Name: Elastase, Human leukocytes Visit Supplier Webpage Request for quotationCAS: 9004-06-2
Tel: 609-228-6898
Email: sales@medchemexpress.com
tech@medchemexpress.com
Mobile: 609-228-6898
Spot supply
Product Name: Elastase from human leukocytes lyophilized powder, >=50 units/mg protein (Bradford) Visit Supplier Webpage Request for quotationCAS: 9004-06-2
Tel: +86-400-900-4166
Email: product@acmec-e.com
Mobile: +86-18621343501
QQ: 2881950922
Wechat: 18621343501
WhatsApp: +86-18621343501
Spot supply
Product Name: Elastase, human leucocytes Visit Supplier Webpage Request for quotationCAS: 9004-06-2
Tel: +86-18821248368
Email: Int06@meryer.com
Mobile: +86-18821248368
QQ: 495145328
WhatsApp: +86-18821248368
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